Using 23S rDNA to identify contaminations of Escherichia coli in Agrobacterium tumefaciens cultures
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URI: http://hdl.handle.net/10317/10905ISBN: 978-84-608-5399-2
DOI: 10.31428/10317/10905
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Tecnología de los AlimentosPatrocinadores
This work was financed by the Fundación Séneca de la Región de Murcia and BIOCARM.Realizado en/con
Universidad Politécnica de CartagenaFecha de publicación
2016Editorial
Universidad Politécnica de CartagenaCita bibliográfica
Manchado Rojo, María; Weiss, Julia Rosl; Egea Gutiérrez-Cortines, Marcos. Using 23S rDNA to identify contaminations of Escherichia coli in Agrobacterium tumefaciens cultures. En: Proceedings of the 4th Workshop on agri-food research: WiA.15. Cartagena: Universidad Politécnica de Cartagena, CRAI Biblioteca, 2016. Pp. 90-92. ISBN: 978-84-608-5399-2Palabras clave
BacteriaGenetic transformation
PCR
Resumen
Cross contamination of Agrobacterium tumefaciens stocks with Escherichia coli are difficult to identify by microbiological techniques, leading to false negative results in transformation experiments. We have developed a genotyping assay for A. tumefaciens and E. coli lab strains based on amplification of 23S rDNA by PCR. Agrobacterium strains LBA 4404, C58 and EHA105 and E. coli DB3.1, DH5α and XL1-Blue can be identified separating the corresponding PCR amplicons in 2.5% agarose gels. However in crossed contaminations, interpretation of results is improved using melting point analysis on a quantitative PCR machine.
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