Genetic relationship among seven specialized forms of Fusarium oxysporum determined by DNA sequencing of the ITS region and AFLPs
Author
Arroyo García, Rosa; Cenis Anadón, José Luis; Tello Marquina, Julio César; Martínez Zapater, José Miguel; Cifuentes Romo, Dina CarmenKnowledge Area
Producción VegetalSponsors
This work was financed by the project INIA SC93-135.Publication date
2003Publisher
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)Bibliographic Citation
ARROYO GARCÍA, R., CENIS, J.L., TELLO, J., MARTÍNEZ ZAPATER, CIFUENTES, D. Genetic relationship among seven specialized forms of Fusarium oxysporum determined by DNA sequencing of the ITS region and AFLPs. Spanish Journal of Agricultural Research, 1 (3): 55-63. ISSN 1695-971-XPeer review
siKeywords
FilogéniaPCR
Hongos
Marcador molecular
Phylogeny
Fungi
Molecular marker
Abstract
[ENG] The fungus Fusarium oxysporum Sch.: Fr. present a high biological and genetic variability, manifested the exixtence of many specialized forms and races. With the goal of evaluating the genetic relationship among different specialized forms, an experiment was carried out involving the application of two types of molecular markers. One consisted of sequencing the internal transcribed spacer (ITS) region of the ribosomal DNA of 17 isolates belonging to seven forms: F.o. melonis (4 isolates), F.o. dianthi (2), F.O. niveum (4), F.o. lycopersici (2), F.o. radicis-lycopersici (3),F.o. lagenaria (1) and F.o. luffae (1). Analysis of these sequences revealed that isolates from different forms presentan identical sequence while isolates of the same form apper distributed in different groups of the dendrogram. This observation was confirmed by an AFLP analysis. In addition to the previously cited forms, ten more were studied in this esperiment, belonging to F.o. ciceris, F.o. cucumerinum, F. ...
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