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dc.contributor.authorWeiss, Julia Rosl 
dc.contributor.authorTerry López, Marta Isabel 
dc.contributor.authorMartos Fuentes, Marina Marta 
dc.contributor.authorLetourneux, Lisa 
dc.contributor.authorRuiz Hernández, María Víctoria 
dc.contributor.authorFernández, Juan A. 
dc.contributor.authorEgea Gutiérrez-Cortines, Marcos 
dc.date.accessioned2019-05-24T06:49:42Z
dc.date.available2019-05-24T06:49:42Z
dc.date.issued2018-02-14
dc.identifier.citationJulia Weiss, Marta I. Terry, Marina Martos-Fuentes, Lisa Letourneux, Victoria Ruiz-Hernández, Juan A. Fernández and Marcos Egea-Cortines. Diel pattern of circadian clock and storage protein gene expression in leaves and during seed filling in cowpea (Vigna unguiculata). En BMC Plant BiologyBMC series – open, inclusive and trusted201818:33es_ES
dc.identifier.issn1471-2229
dc.description.abstractBackground Cowpea (Vigna unguiculata) is an important source of protein supply for animal and human nutrition. The major storage globulins VICILIN and LEGUMIN (LEG) are synthesized from several genes including LEGA, LEGB, LEGJ and CVC (CONVICILIN). The current hypothesis is that the plant circadian core clock genes are conserved in a wide array of species and that primary metabolism is to a large extent controlled by the plant circadian clock. Our aim was to investigate a possible link between gene expression of storage proteins and the circadian clock. Results We identified cowpea orthologues of the core clock genes VunLHY, VunTOC1, VunGI and VunELF3, the protein storage genes VunLEG, VunLEGJ, and VunCVC as well as nine candidate reference genes used in RT-PCR. ELONGATION FACTOR 1-A (ELF1A) resulted the most suitable reference gene. The clock genes VunELF3, VunGI, VunTOC1 and VunLHY showed a rhythmic expression profile in leaves with a typical evening/night and morning/midday phased expression. The diel patterns were not completely robust and only VungGI and VungELF3 retained a rhythmic pattern under free running conditions of darkness. Under field conditions, rhythmicity and phasing apparently faded during early pod and seed development and was regained in ripening pods for VunTOC1 and VunLHY. Mature seeds showed a rhythmic expression of VunGI resembling leaf tissue under controlled growth chamber conditions. Comparing time windows during developmental stages we found that VunCVC and VunLEG were significantly down regulated during the night in mature pods as compared to intermediate ripe pods, while changes in seeds were non-significant due to high variance. The rhythmic expression under field conditions was lost under growth chamber conditions. Conclusions The core clock gene network is conserved in cowpea leaves showing a robust diel expression pattern except VunELF3 under growth chamber conditions. There appears to be a clock transcriptional reprogramming in pods and seeds compared to leaves. Storage protein deposition may be circadian regulated under field conditions but the strong environmental signals are not met under artificial growth conditions. Diel expression pattern in field conditions may result in better usage of energy for protein storage.es_ES
dc.description.sponsorshipThis work was supported by the 7th Research Framework Programme of the European Union “Eurolegume (Enhancing of Legumes Growing in Europe through Sustainable Cropping for Protein Supply for Food and Feed)” FP7– 613781. The funding body had no role in the experimental design, analysis or results shown in the manuscript.es_ES
dc.formatapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherBMCes_ES
dc.relation.urihttps://bmcplantbiol.biomedcentral.com/track/pdf/10.1186/s12870-018-1244-2es_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights© The Author(s). 2018
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.titleDiel pattern of circadian clock and storage protein gene expression in leaves and during seed filling in cowpea (Vigna unguiculata)es_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.subjectStorage proteinses_ES
dc.subjectCircadian rhythmes_ES
dc.subjectLHYes_ES
dc.subjectELF3es_ES
dc.subjectTOC1es_ES
dc.subjectLeguminees_ES
dc.subjectConvicilines_ES
dc.subject.otherGenéticaes_ES
dc.identifier.urihttp://hdl.handle.net/10317/7775
dc.peerreviewSies_ES
dc.identifier.doi10.1186/s12870-018-1244-2
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.type.versioninfo:eu-repo/semantics/publishedVersiones_ES
dc.subject.unesco2409 Genética
dc.contributor.funderComisión Europeaes_ES


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