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dc.contributor.authorGrant, Charles 
dc.contributor.authorJacobson, Rob 
dc.contributor.authorIlias, Aris 
dc.contributor.authorBerger, Madeleine 
dc.contributor.authorVasakis, Emmanouil 
dc.contributor.authorBielza Lino, Pablo 
dc.contributor.authorZimmer, Christoph T. 
dc.contributor.authorWilliamson, Martin S. 
dc.contributor.authorFfrench-Constant, Richard H. 
dc.contributor.authorVontas, John 
dc.contributor.authorRoditakis, Emmanouil 
dc.contributor.authorBass, Chris 
dc.date.accessioned2021-06-21T06:15:01Z
dc.date.available2021-06-21T06:15:01Z
dc.date.issued2019-03-25
dc.identifier.issn1526-498X
dc.description.abstractBACKGROUND The tomato leafminer, Tuta absoluta, is an economically important pest of tomatoes in Europe, Africa, Asia and South America. In the UK this species is controlled using an integrated pest management (IPM) programme which incorporates the insecticides spinosad and chlorantraniliprole. In response to UK grower concerns of loss of efficacy of these compounds at certain sites, insecticide bioassays were performed on five populations collected from four commercial glasshouses and potential mechanisms of resistance investigated. RESULTS We observed high levels of resistance to spinosad in four of the strains, and in two of these tolerance to chlorantraniliprole. Selection of one of these strains with chlorantraniliprole rapidly resulted in a line exhibiting potent resistance to this compound. Sequencing of messenger RNA encoding the nicotinic acetylcholine receptor (nAChR) alpha 6 subunit, target of spinosad, revealed Ta alpha 6 transcripts in the spinosad-resistant strains that lack exon 4 and encode a highly truncated protein, or contain a triplet deletion in the predicted first transmembrane domain resulting in the loss of a highly conserved amino acid. Sequencing of the ryanodine receptor gene, encoding the target of diamide insecticides, of the chlorantraniliprole-selected line revealed an amino acid substitution (G4903V) that has been previously linked to diamide resistance in populations of T. absoluta in the Mediterranean and South America. CONCLUSION Taken together our results reveal emerging resistance in UK populations of T. absoluta to two of the most important insecticides used as part of IPM, with significant implications for the control of this species in the UK. (c) 2019 Society of Chemical Industryes_ES
dc.description.sponsorshipThis work was funded by PhD studentship awards from the Agriculture and Horticulture Development Board (AHDB) (CP 162) and the Biotechnology and Biological Sciences Research Council (BBSRC) (grant number: 1096240). ER was supported by funding from the European Union's Seventh Framework Programme for research, technological development and demonstration under the STomP project, grant agreement no. 219262 (ARIMNet).es_ES
dc.formatapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherWILEYes_ES
dc.relation.urihttps://onlinelibrary.wiley.com/doi/full/10.1002/ps.5381es_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.titleThe evolution of multiple-insecticide resistance in UK populations of tomato leafminer, Tuta absolutaes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.subjectSpinosades_ES
dc.subjectChlorantraniliprolees_ES
dc.subjectResistancees_ES
dc.subjectTomato leafmineres_ES
dc.subject.otherFisiología Vegetales_ES
dc.subject.otherProducción Vegetales_ES
dc.identifier.urihttp://hdl.handle.net/10317/9462
dc.peerreviewSies_ES
dc.identifier.doi10.1002/ps.5381
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.type.versioninfo:eu-repo/semantics/publishedVersiones_ES
dc.subject.unesco2413 Biología de Insectos (Entomología)es_ES
dc.subject.unesco2413.01 Entomología Generales_ES
dc.subject.unesco5102.01 Agriculturaes_ES
dc.contributor.convenianteUniversidad Politécnica de Cartagenaes_ES
dc.contributor.convenianteUniversidad de Exeteres_ES


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