Genetic relationship among seven specialized forms of Fusarium oxysporum determined by DNA sequencing of the ITS region and AFLPs
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AuthorArroyo García, Rosa; Cenis Anadón, José Luis; Tello Marquina, Julio César; Martínez Zapater, José Miguel; Cifuentes Romo, Dina Carmen
Knowledge AreaProducción Vegetal
SponsorsThis work was financed by the project INIA SC93-135.
PublisherInstituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
Bibliographic CitationARROYO GARCÍA, R., CENIS, J.L., TELLO, J., MARTÍNEZ ZAPATER, CIFUENTES, D. Genetic relationship among seven specialized forms of Fusarium oxysporum determined by DNA sequencing of the ITS region and AFLPs. Spanish Journal of Agricultural Research, 1 (3): 55-63. ISSN 1695-971-X
[ENG] The fungus Fusarium oxysporum Sch.: Fr. present a high biological and genetic variability, manifested the exixtence of many specialized forms and races. With the goal of evaluating the genetic relationship among different specialized forms, an experiment was carried out involving the application of two types of molecular markers. One consisted of sequencing the internal transcribed spacer (ITS) region of the ribosomal DNA of 17 isolates belonging to seven forms: F.o. melonis (4 isolates), F.o. dianthi (2), F.O. niveum (4), F.o. lycopersici (2), F.o. radicis-lycopersici (3),F.o. lagenaria (1) and F.o. luffae (1). Analysis of these sequences revealed that isolates from different forms presentan identical sequence while isolates of the same form apper distributed in different groups of the dendrogram. This observation was confirmed by an AFLP analysis. In addition to the previously cited forms, ten more were studied in this esperiment, belonging to F.o. ciceris, F.o. cucumerinum, F. ...
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