Quantitative evaluation of bias in barcode markers derived from complex samples
Ver/
Identificadores
URI: http://hdl.handle.net/10317/10825ISBN: 978-84-697-1358-7
DOI: 10.31428/10317/10825
Compartir
Estadísticas
Ver Estadísticas de usoMetadatos
Mostrar el registro completo del ítemAutor
Pawluczyk, Marta; Weiss, Julia Rosl; Links, Matthew G.; Egaña Aranguren, Mikel; Wilkinson, Mark; [et al.]Área de conocimiento
Tecnología de los AlimentosPatrocinadores
This work was funded by the Comunidad Autónoma de la Región de Murcia Project “Molecular markers in coservation and management of the flora of Murcia Region”.Realizado en/con
Universidad Politécnica de CartagenaFecha de publicación
2014Editorial
Universidad Politécnica de CartagenaCita bibliográfica
PAWLUCZY, Marta et al. Quantitative evaluation of bias in barcode markers derived from complex samples. En: III Workshop en Investigación Agroalimentaria: WiA3.14. Cartagena: Universidad Politécnica de Cartagena, Servicio de Documentación, 2014. Pp. 78-81. ISBN: 978-84-697-1358-7Palabras clave
WiAMeta‐barcoding
Next Generation Sequencing
Ion torrent
PCR efficiency
Resumen
PCR products have become a major commodity used to identify organisms based on
polymorphism at the DNA level. One problem arising is that unbiased identification of
organisms takes as working hypothesis that when DNA is extracted from a sample, a
positive signal will be obtained if universal primers are used and DNA quality is suitable
for PCR. As this assumption is not always correct we used a system where large
differences in PCR success have been described to identify where biases appear and
maybe identify solutions. Plants can be identified with at least seven independent
plastid‐located loci. These differ in their degree of PCR success and how informative
they are in terms of taxonomically useful sequence polymorphisms. Here we used six
common plastid loci spanning 48 plant species and performed a quantitative analysis
of bias at each step of the identification process. As expected we found important
differences in PCR efficiency within a single species, depending on the ...
El ítem tiene asociados los siguientes ficheros de licencia:
Redes sociales