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dc.contributor.authorHuertas Baquero, Juan Pablo 
dc.contributor.authorRos, M. 
dc.contributor.authorEsteban, M. 
dc.contributor.authorPalop Gómez, Alfredo 
dc.contributor.authorHill, Colin 
dc.contributor.authorÁlvarez, A.
dc.identifier.citationHUERTAS BAQUERO, Juan Pablo et al. Determination of genes involved in heat resistance response of Cronobacter sakazakii. En: III Workshop en Investigación Agroalimentaria: WiA3.14. Cartagena: Universidad Politécnica de Cartagena, Servicio de Documentación, 2014. Pp. 22-25. ISBN: 978-84-697-1358-7es_ES
dc.description.abstractCronobacter sakazakii is considered an emerging opportunistic pathogen causing meningitis, septicaemia and enterocilitis in neonates, related to the use of contaminated Powdered Infant Formula (PIF). C. sakazakii has an unusual surviving ability under dry conditions and has been suggested to be one of the most thermotolerant members of the Enterobacteriaceae. It is necessary to understand the molecular mechanisms underlying the thermal resistance of C. sakazakii and may ultimately be useful in the development of control strategies in PIF factories. In the current study, a transposon mutagenesis approach was used to identify the genes involved in heat resistance. A total of 23 mutants were found corresponding to 12 different defective genes. Heat resistance of selected mutants were determined with the use of the thermoresistometer Mastia. Only 2 mutants had a greater sensitivity to heat compared with the heat resistance of the wild type. Genes identified to be involved in the cellular response to thermal treatments were Ribosome maturation protein RimP and Outer membrane Porin L (OmpL). The results suggest that the novo protein synthesis, and the use of cysteine for the formation of disulfide bonds for stabilization of proteins against denaturation during thermal treatments are key processes in the resistance against heat stress.es_ES
dc.description.sponsorshipThe financial support of this research was provided by the Ministry of Science and Technology of the Spanish Government and European Regional Development Fund (ERDF) through Project AGL‐ 2010‐19775. J.P Huertas is grateful to the Spanish Ministry of Science and Innovation for his fellowship (BES‐2011‐046580). We acknowledge the funding received by Food for Health, Ireland under the grant number CC20080001 by Enterprise Ireland.es_ES
dc.publisherUniversidad Politécnica de Cartagenaes_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España
dc.titleDetermination of genes involved in heat resistance response of Cronobacter sakazakiies_ES
dc.subject.otherTecnología de los Alimentoses_ES
dc.subjectC. sakazakiies_ES
dc.subjectHeat resistancees_ES
dc.subjectTransposon mutagenesises_ES
dc.subjectHeat responsees_ES
dc.subjectWeibull distributiones_ES
dc.contributor.convenianteUniversidad Politécnica de Cartagenaes_ES
dc.conference.titleActas del III Workshop en Investigación Agroalimentaria. WiA 3.1
dc.conference.publisherUniversidad Politécnica de Cartagena

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Atribución-NoComercial-SinDerivadas 3.0 España
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