A comparison of semi‑quantitative methods suitable for establishing volatile profles
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AuthorRuiz Hernández, María Victoria; Roca Hernández, María José; Egea Gutiérrez-Cortines, Marcos; Weiss, Julia Rosl
SponsorsThis work was supported by Ministerio de Economía y Competitividad BFU2017-88300-C2-1R, Fundación Séneca 19398/PI/14 and by the Ministerio de Educación Cultura y Deporte (FPU13/03606) to VRH.
Bibliographic CitationRuiz-Hernández, V., Roca, M.J., Egea-Cortines, M. et al. A comparison of semi-quantitative methods suitable for establishing volatile profiles. Plant Methods 14, 67 (2018). https://doi.org/10.1186/s13007-018-0335-2
Background: Full scent profles emitted by living tissues can be screened by using total ion chromatograms gener ated in full scan mode and gas chromatography–mass spectrometry technique using Headspace Sorptive Extraction. This allows the identifcation of specifc compounds and their absolute quantifcation or relative abundance. Quanti fcations ideally should be based on calibration curves using standards for each compound. However, the unpredict able composition of Volatile Organic Compounds (VOCs) and lack of standards make this approach difcult. Research ers studying scent profles therefore concentrate on identifying specifc scent footprints i.e. relative abundance rather than absolute quantities. We compared several semi-quantitative methods: external calibration curves generated in the sampling system and by liquid addition of standards to stir bars, total integrated peak area per fresh weight (FW), normalized peak area per FW, semi-quantifcation based on internal standard ...
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