Immobilization in ionogel: a newway to improve the activity and stability of candida antarctica lipase B
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Escudero Solano, Alfonso; Pérez de los Ríos, Antonia; Godínez Seoane, Carlos; Tomás, Francisca; Hernández Fernández, Francisco JoséGrupo de investigación
INQUIPROÁrea de conocimiento
Ingeniería QuímicaPatrocinadores
This research was partially supported by the Spanish Ministry of Economy and Competitiveness (MINECO) (Grant number: RTI2018-099011-B-I00) and Séneca Foundation (Grant number: 20957/PI/18)Fecha de publicación
2020-07-15Editorial
MDPICita bibliográfica
Escudero, A.; de los Ríos, A.P.; Godínez, C.; Tomás, F.; Hernández-Fernández, F.J. Immobilization in Ionogel: A New Way to Improve the Activity and Stability of Candida antarctica Lipase B. Molecules 2020, 25, 3233. https://doi.org/10.3390/molecules25143233Revisión por pares
siPalabras clave
Enzymatic immobilizationOrganogels
Ionogels
Ionic liquid
Ester synthesis
Enzyme
Green chemistry
Resumen
New Candida antarctica lipase B derivatives with higher activity than the free enzyme
were obtained by occlusion in an organogel of an ionic liquid (ionogel) based on the ionic liquid
[Omim][PF6] and polyvinyl chloride. The inclusion of glutaraldehyde as a crosslinker improved the
properties of the ionogel, allowing the enzymatic derivative to reach 5-fold higher activity than the free
enzyme and also allowing it to be reused at 70 ◦C. The new methodology allows enzymatic derivatives
to be designed by changing the ionic liquid, thus providing a suitable microenvironment for the
enzyme. The ionic liquid may act on substrates to increase their local concentration, while reducing
water activity in the enzyme’s microenvironment. All this allows the activity and selectivity of the
enzyme to be improved and greener processes to be developed. The chemical composition and
morphology of the ionogel were also studied by scanning electron microscopy–energy dispersive
X-ray spectroscopy, ...
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