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dc.contributor.authorNiñirola Campoy, Diana 
dc.date.accessioned2018-04-05T15:31:09Z
dc.date.available2018-04-05T15:31:09Z
dc.date.issued2015
dc.description.abstract[ENG] The floating system is one of the easiest and cheapest hydroponic methods used to produce baby leaf vegetables, a product which has grown in popularity in recent years as a ready-to-eat vegetable included in salads or as a single products. The aim of this thesis was to assess the influence of three levels of aeration of the nutrient solution, the growing cycle or the application of plant growth promoting rhizobacteria (PGPR) on yield, on quality and on shelf life as a fresh-cut product of different species of baby leaf vegetables. In the experiments of aeration of the nutrient solution, three levels [no aeration (NA), low aeration (LA) or high aeration (HA)] were studied in three different species (purslane, watercress and lettuce). The study of three levels of aeration of the nutrient solution on the growth and quality of two cultivars of purslane (Portulaca oleracea L.) over four crop cycles showed that purslane exhibited little sensitivity to oxygen depletion in the rooting medium, since it was able to adapt to a gradual reduction in oxygen content. Under such conditions, purslane plants created an aerenchyma tissue that helped to maintain growth. Under conditions in which no aeration was provided, there was a slight decrease in plant growth. The final quality of the product was improved because leaf nitrate concentrations were reduced compared with the high aeration treatment, and the content of functional phytochemicals and chlorophyll contents were increased. The study of the effects of nutrient solution aeration and growing cycle (spring vs. winter) on yield, quality and on shelf life as a fresh-cut product of watercress (Nasturtium officinale R. Br.) showed that in the spring cycle, the plants had significantly higher yield and antioxidant capacity and lower specific leaf area, total root length, root diameter, length of 0 to 0.5 mm diameter root, and oxalate content than in the winter cycle. The absence of aeration increased the antioxidant capacity and vitamin C content in both cycles. Several adventitious roots developed exogenously from the watercress stem at the nodes as a morphological adaptation to oxygen depletion, particularly in NA conditions. The nitrate, oxalate, Ca2+, K+ contents, and microbial populations were affected by both the cycle and the aeration conditions. Hue angle of the leaves was affected by both the cycle and storage time, and chromaticity and lightness were affected by the three factors (cycle, aeration, and storage time). The global quality was significantly higher (7.8 over 9 points hedonic scale) in the spring cycle than in winter, the score reflecting their marketable value (7.0 over 9 points). The mild dehydration problems observed in the winter cycle that led to a slightly lower overall product quality that could be the result of the development of thinner leaves and also the differences in the respiration rates compared with the spring cycle. In general, the spring cycle led to higher productivity, antioxidant capacity, and Ca2+ and K+ contents and lower oxalate content. Aeration slightly affected the quality of the final product, the plants grown in non-aerated conditions being richer in vitamin C and antioxidants and with lower nitrate content. The study of the effects of nutrient solution aeration and growing cycle (autumn, winter and summer) on yield, quality, and on shelf life as a fresh-cut product of a red lettuce (Lactuca sativa L.) showed that the specific leaf area was lowest in winter. Yield was affected only by the growing cycle, showing the highest value in autumn. Lack of aeration produced shorter total root length but did not affect the root diameter. The percentage of dry matter and the nitrate content were affected by growing cycle and aeration, total phenolics and mesophilic microorganism by both aeration and storage time, hue angle and chromacity by growing cycle and storage time, and antioxidant capacity, vitamin C, lightness and psychrophilic microorganisms were affected by all three factors. NA conditions increased the antioxidant capacity in summer and vitamin C content in winter. After 7 days of storage at 5 ºC, the antioxidant capacity, total phenolics, vitamin C and nitrate content decreased. The leaves were redder (higher Hue angle) in autumn and winter. The lowest mesophilic and psychrophilic count was observed in autumn. The last two chapters relate to the use of plant growth promoting rhizobacteria (PGPR) to improve the quality and yield of baby leaf vegetables grown in floating system. Regarding to the effect of application of two PGPR (Bacillus subtilis and Bacillus velezensis) and two concentrations of nitrogen (4 and 12 mM) in the nutrient solution on yield, quality and nitrate content of two baby leaf lettuce cultivars the results showed that in both cultivars and crop cycles plant height was affected by the level of N and the PGPR application, while leaf area, fresh and dry weight were only affected by the level of N. The use of the nutrient solution containing 12 mM of nitrogen increased the accumulation of nitrate in leaves. The application of B. velezensis in the nutrient solution provoked a decrease of nitrate content in red lettuce leaves respect to control. Finally, root growth was not affected by neither nutritive solution nor bacterial inoculants in both cultivars. Finally, the study of the effect of applying a PGPR (Bacillus subtilis) on the yield, quality and safety of watercress considering two factors: substrate disinfection and inoculation with B. subtilis showed that substrate disinfection had a positive effect on plant development because it increased the shoot antioxidant capacity and general plant growth and decreased the colony-forming units of moulds. In turn, inoculation with B. subtilis increased the antioxidant capacity but decreased the chlorophyll a, chlorophyll b and carotenoid contents and did not affect the rest of parameters measured.es_ES
dc.formatapplication/pdfes_ES
dc.language.isospaes_ES
dc.publisherDiana Niñirola Campoyes_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.titleEffects of aeration of the nutrient solution and application of PGPR on the production and quality of baby leaf vegetables grown in floating systemes_ES
dc.typeinfo:eu-repo/semantics/doctoralThesises_ES
dc.subject.otherProducción Vegetales_ES
dc.contributor.advisorFernández Hernández, Juan Antonio 
dc.contributor.advisorEgea Gilabert, Catalina 
dc.date.submitted2015-04-09
dc.subjectProducción de Cultivoses_ES
dc.subjectTécnicas de cultivoes_ES
dc.subjectPlantas ornamentaleses_ES
dc.subjectBaby leaf vegetablees_ES
dc.subjectCultivo hidropónicoes_ES
dc.identifier.urihttp://hdl.handle.net/10317/6801
dc.description.centroEscuela Internacional de Doctoradoes_ES
dc.contributor.departmentProducción Vegetales_ES
dc.identifier.doi10.31428/10317/6801
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.description.universityUniversidad Politécnica de Cartagenaes_ES
dc.subject.unesco3103.01 Producción de Cultivoses_ES
dc.subject.unesco3103.05 Técnicas de Cultivoes_ES
dc.description.programadoctoradoPrograma de Doctorado en Técnicas Avanzadas en Investigación y Desarrollo Agrario y Alimentarioes_ES


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